bladder transitional cell carcinoma  (DSMZ)

Journal: bioRxiv

Article Title: Rapid evolution of Klebsiella pneumoniae biofilms in vitro delineates adaptive changes selected during infection

doi: 10.1101/2024.03.16.585345

Figure Lengend Snippet: a, Predicted MrkD protein structure (PDB K7R402) with marked mutated positions, b, Evolutionary trajectories in the IA565-SF-4 lineage. Biofilm capacity refers to the conditions during evolution (silicone with fibrinogen), c, CV-stained biofilm biomass on silicone pegs, silicone + fibrinogen pegs, and polystyrene wells after 48 h growth in evolved mrkD mutants. Heat map values illustrate medians of at least four biological replicates, d, Attachment to pegs after 3 h of growth. Results illustrate eight biological replicates with medians and 95% Cl. Comparisons between parental strains and mutants were made by the Kruskal-Wallis test, followed by Dunn’s multiple comparison test for IA565 and the Mann-Whitney test for C3091 and DA14734, with significance at ρ<0.05. e , Attachment of exponentially grown bacteria to A549 lung and T24 bladder epithelial cells. Bars show a median of three independent experiments with two technical replicates. Statistical significance was assessed by one-way ANOVA followed by Dunnett’s multiple comparison test; results were considered significant when p<0.05. f , SEM images of parental C3091 (left) and mrkD mutants (middle and right) grown for 16 h on silicone-coated pegs.

Article Snippet: T24 bladder transitional carcinoma cells (ATCC HTB-4) were grown in McCoy’s 5a Medium Modified (Sigma) supplemented with 10% heat-inactivated fetal bovine serum (FBS).

Techniques: Staining, Comparison, MANN-WHITNEY, Bacteria